منابع مشابه
Engineering subtilisin into a fluoride-triggered processing protease useful for one-step protein purification.
Subtilisin was engineered into a highly specific, processing protease, and the subtilisin prodomain was coengineered into an optimized recognition sequence. This involved five steps. First, a robust subtilisin mutant was created, which could tolerate the subsequent mutations needed for high specificity. Second, the substrate binding pocket was mutated to increase its sequence selectivity. Third...
متن کاملHomology modelling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteinases.
Subtilases are members of the family of subtilisin-like serine proteases. Presently, greater than 50 subtilases are known, greater than 40 of which with their complete amino acid sequences. We have compared these sequences and the available three-dimensional structures (subtilisin BPN', subtilisin Carlsberg, thermitase and proteinase K). The mature enzymes contain up to 1775 residues, with N-te...
متن کاملNanocomposite Based on Albumin Protein and Review of Its Applications in Medical Engineering
Albumin is a protein macromolecule that it has been attractive since the early 20th century and has several therapeutic applications. Albumin is an aboundant serum with good biocompatibility, hematopoiesis and biodegradability in blood. The existence of multiple functional groups makes it possible to carry significant amounts of therapeutic and diagnostic factors, as well as targeting factors. ...
متن کاملDeletional Protein Engineering Based on Stable Fold
Diversification of protein sequence-structure space is a major concern in protein engineering. Deletion mutagenesis can generate a protein sequence-structure space different from substitution mutagenesis mediated space, but it has not been widely used in protein engineering compared to substitution mutagenesis, because it causes a relatively huge range of structural perturbations of target prot...
متن کاملProtein engineering.
The prospects for protein engineering, including the roles of x-ray crystallography, chemical synthesis of DNA, and computer modelling of protein structure and folding, are discussed. It is now possible to attempt to modify many different properties of proteins by combining information on crystal structure and protein chemistry with artificial gene synthesis. Such techniques offer the potential...
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ژورنال
عنوان ژورنال: Journal of the agricultural chemical society of Japan
سال: 1991
ISSN: 0002-1407,1883-6844
DOI: 10.1271/nogeikagaku1924.65.63